Invited Talk World Congress DSA 2012

Quantitative Measurement of Cellular Events

Hualan Zhang
Institute of Computer Vision and applied Computer Sciences, IBaI, Leipzig, Germany, Project Consortium QuantPro


Cellular assays are highly recommended scientific tools in modern drug research and examination of pathological processes on cellular level.
Within the project QuantPro we developed based on innovative cell-based microscopy end-point assays a robust quantitative reference model for better understanding or quantitative measurements of dynamic or time dependent events in three key disease-relevant cellular pathways involving either internalisation (cell invasion or endocytosis of viruses or bacterial pathogens) or internal movements (mitochondria). Furthermore, we developed a suitable complex cellular functional experimental setup for drug screening in oncology which allows for microtiter plate-based screening modalities, multiple wavelength operation and high-resolution imaging. A highly innovative technology component for the application of a newly developed imaging system “Selective Plane Illumination Microscope (SPIM)” to extend the dynamic measurements made by two-dimensional microscopy and confocal scanning sections to 3D microscopy by confocal theta microscopy. Novel bioinformatic and software solutions were develped to evaluate both cellular models in a dynamic 3D mode. The talk summarizes the results of the project QuantPro. The project consortium has been comprised of five working groups MPI-CBG Dresden, Schering AG, EMBL group Stelzer, Institute of Computer Vision and applied Computer Sciences, IBaI, and EMBL group Bork.